| 產品編號 | bs-55176R |
| 英文名稱 | [KO驗證抗體] PRKAR2A |
| 中文名稱 | 蛋白激酶A受體2α亞基抗體 |
| 別 名 | KAP2; KAP2_HUMAN; MGC3606; PKR 2; PKR2; PRKA R2; PRKAR 2; PRKAR2; Protein kinase A RII alpha subunit; Protein kinase cAMP dependent regulatory type II alpha; cAMP dependent protein kinase regulatory subunit alpha 2; cAMP dependent protein kinase regulatory subunit RII alpha; cAMP dependent protein kinase type II alpha regulatory chain; cAMP dependent protein kinase type II alpha regulatory subunit; cAMP-dependent protein kinase type II-alpha regulatory subunit. |
| 研究領域 | 腫瘤 細胞生物 免疫學 轉錄調節因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human, Mouse, Rat, |
| 產品應用 | WB=1:1000-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 49kDa |
| 細胞定位 | 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant human PRKAR2A: 1-404/404 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The second messenger cyclic AMP (cAMP) mediates diverse cellular responses to external signals such as proliferation, ion transport, regulation of metabolism and gene transcription by activation of the cAMP-dependent protein kinase (cAPK or PKA). Activation of PKA occurs when cAMP binds to the two regulatory subunits of the tetrameric PKA holoenzyme, resulting in release of active catalytic subunits. Activation of transcription upon elevation of cAMP levels results from translocation of PKA to the nucleus, where it phosphorylates the transcription factor cAMP response element binding protein (CREB) on Serine 133, which in turn leads to TFIIB binding to TATA-box-binding protein TBP1, thus linking phospho-CREB to the Pol II transcription initiation complex. Mouse Serine 96 (designated Ser 99 in human) is a phosphorylation site on the PKA II?regulatory subunit Function: Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase. Subunit: The inactive form of the enzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP produces two active catalytic monomers and a regulatory dimer that binds four cAMP molecules. Interacts with AKAP4 and CBFA2T3. Interacts with the phosphorylated form of PJA2. Subcellular Location: Cytoplasm. Cell membrane. Note=Co-localizes with PJA2 in the cytoplasm and the cell membrane. Tissue Specificity: Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible. Post-translational modifications: Phosphorylated by the activated catalytic chain. Similarity: Belongs to the cAMP-dependent kinase regulatory chain family. SWISS: P13861 Gene ID: 5576 Database links: SwissProt: P13861 Human SwissProt: P12367 Mouse Unigene: 631923 Human Unigene: 731573 Human Unigene: 253102 Mouse Unigene: 486392 Mouse Unigene: 9742 Rat |
| 產品圖片 |
Sample:
Lane 1: Hela (Human) Cell Lysate at 25 ug Lane 2: PRKAR2A knockout (KO) Hela (Human) Cell Lysate at 25 ug Primary: Anti-PRKAR2A (bs-55176R) at 1/1000 dilution Secondary: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution Predicted band size: 46 kD Observed band size: 46 kD 
Sample:
Lane 1: Lymph node (Mouse) Lysate at 40 ug Lane 2: Testis (Mouse) Lysate at 40 ug Lane 3: Testis (Rat) Lysate at 40 ug Lane 4: Cerebellum (Mouse) Lysate at 40 ug Lane 5: K562 (Human) Cell Lysate at 30 ug Lane 6: Hela (Human) Cell Lysate at 30 ug Lane 7: MDA-MB-231 (Human) Cell Lysate at 30 ug Lane 8: U251 (Human) Cell Lysate at 30 ug Lane 9: U937 (Human) Cell Lysate at 30 ug Lane 10: A673 (Human) Cell Lysate at 30 ug Primary: Anti-PRKAR2A (bs-55176R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 46 kD Observed band size: 46 kD |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
