| 產品編號 | bs-55193R |
| 英文名稱 | [KO驗證抗體] SCD |
| 中文名稱 | 固醇酰輔酶A脫氫酶1抗體 |
| 別 名 | ACOD_HUMAN; Acyl-CoA desaturase; Delta(9)-desaturase; Delta-9 desaturase; Delta-9-Desaturase; FADS5; Fatty acid desaturase; PRO0998; SCD 1; SCD; SCD1; Stearoyl-CoA Desaturase. |
| 研究領域 | 腫瘤 心血管 免疫學 信號轉導 合成與降解 脂蛋白 新陳代謝 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human, Mouse, (predicted: Rat, ) |
| 產品應用 | WB=1:500-2000 IHC-P=1:50-100 IHC-F=1:50-100 ICC=1:100 IF=1:50-100 （石蠟切片需做抗原修復）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 41kDa |
| 細胞定位 | 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SCD: 200-359/359 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Stearoyl-CoA desaturase (SCD) is a regulatory enzyme in lipogenesis. The SCD1 gene is tightly regulated by signals such as insulin, leptin, carbohydrate, fatty acids and temperature. The enzyme is constitutively expressed in adipose tissue and is induced in liver in response to a fat free, high carbohydrate diet. SCD1 expression is higher in female than male mouse. The repression of RNA levels and enzyme activity of hepatic SCD1 by Leptin has been shown to significantly reduce the triglyceride storage, VLDL production and therefore an increase in fatty acid oxidation. Inhibition of SCD1 could be of benefit for treatment of obesity, hepatic steatosis and other metabolic disorders. Function: Terminal component of the liver microsomal stearyl-CoA desaturase system, that utilizes O(2) and electrons from reduced cytochrome b5 to catalyze the insertion of a double bond into a spectrum of fatty acyl-CoA substrates including palmitoyl-CoA and stearoyl-CoA. Subcellular Location: Endoplasmic reticulum membrane; Multi-pass membrane protein (Probable). Similarity: Belongs to the fatty acid desaturase family. SWISS: O00767 Gene ID: 6319 Database links: Entrez Gene: 6319 Human Entrez Gene: 20249 Mouse Omim: 604031 Human SwissProt: O00767 Human SwissProt: P13516 Mouse Unigene: 558396 Human Unigene: 597496 Human 固醇酰輔酶A脫氫酶1(SCD1)是催化細胞中飽和脂肪酸(SFA)轉化為單不飽和脂肪酸(MUFA)的限速酶，在調控脂肪酸代謝、維持能量平衡、調節胰島素敏感性及衰老等方面都發揮著重要作用，近來在肥胖、激素調節、對瘦素的作用等研究受到了重視。 |
| 產品圖片 |
Sample:
Lane 1: Hela (Human) Cell Lysate at 25 ug Lane 2: SCD knockout (KO) Hela (Human) Cell Lysate at 25 ug Primary: Anti-RHOC (bs-55193R) at 1/1000 dilution Secondary: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution Predicted band size: 38 kD Observed band size: 38 kD 
Sample:
Lane 1: HepG2 (Human) Cell Lysate at 30 ug Lane 2: MCF-7 (Human) Cell Lysate at 30 ug Primary: Anti-SCD (bs-55193R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 38 kD Observed band size: 38 kD 
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SCD) Polyclonal Antibody, Unconjugated (bs-55193R) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SCD) Polyclonal Antibody, Unconjugated (bs-55193R) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
U2-OS cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (KO Validated)SCD polyclonal Antibody, Unconjugated (bs-55193R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
