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    [KO驗證抗體] Rabbit Anti-Beclin 1  antibody (bs-55024R)
    ~~~促銷,代碼SSY221101~~~
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    產品編號 bs-55024R
    英文名稱 [KO驗證抗體] Beclin 1
    中文名稱 自噬效應蛋白Beclin 1抗體
    別    名 Beclin-1; ATG6; beclin1; VPS30; Beclin 1 like protein 1; 4921513J16Rik; 5430417M23Rik; ATG6; BECLIN 1; BECN1; MGC6843; BECN1_HUMAN.  
    研究領域 腫瘤  免疫學  神經生物學  細胞凋亡  
    抗體來源 Rabbit
    克隆類型 Polyclonal
    交叉反應 Human, Mouse, Rat, 
    產品應用 WB=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100 IF=1:100-500 (石蠟切片需做抗原修復)
    not yet tested in other applications.
    optimal dilutions/concentrations should be determined by the end user.
    理論分子量 60kDa
    細胞定位 細胞漿 細胞膜 
    性    狀 Liquid
    濃    度 1mg/ml
    免 疫 原 KLH conjugated synthetic peptide derived from human BECN1: 181-280/450 
    亞    型 IgG
    純化方法 affinity purified by Protein A
    緩 沖 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
    保存條件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
    注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    PubMed PubMed
    產品介紹 Beclin 1 is the first identified mammalian gene to mediate autophagy and also has tumor suppressor and antiviral function. Autophagy, a process of bulk protein degradation through an autophagosomic lysosomal pathway, is important for differentiation, survival during nutrient deprivation, and normal growth control, and is often defective in tumor cells. Beclin 1 was originally isolated in a yeast two hybrid screen to identify Bcl 2 binding partners and maps to a tumor susceptibility locus on human chromosome 17q21 that is frequently monoallelically deleted in human breast, ovarian and prostate cancer. Beclin 1 encodes an evolutionarily conserved 52kDa coiled coil protein that is expressed in human muscle, epithelial cells and neurons.

    Function:
    Plays a central role in autophagy. Required for the abcission step in cytokinesis. May play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus.

    Subunit:
    Interacts with GOPC and GRID2. Interacts with AMBRA1. Forms a complex with PIK3C3 and PIK3R4 and either UVRAG and KIAA0226/Rubicon, or with ATG14. UVRAG and ATG14 form mutually exclusive complexes through direct competition for BECN1-binding. The complex containing ATG14 up-regulates autophagy, while the one containing Rubicon down-regulates autophagy. Interacts with BCL2 and BCL2L1. Interacts with VMP1. Interacts with PIK3CB. Interacts with DAPK1. Interacts with human cytomegalovirus/HHV-5 protein TRS1.

    Subcellular Location:
    Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein. Note=Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells.

    Tissue Specificity:
    Ubiquitous.

    Post-translational modifications:
    Phosphorylation at Thr-119 by DAPK1 reduces its interaction with BCL2 and BCL2L1 and promotes induction of autophagy.
    Ubiquitinated. Deubiquitinated by USP10 and USP13, leading to stabilize the PIK3C3/VPS34-containing complexes.

    Similarity:
    Belongs to the beclin family.

    SWISS:
    Q14457

    Gene ID:
    8678

    Database links:

    Entrez Gene: 8678 Human

    Entrez Gene: 56208 Mouse

    Entrez Gene: 114558 Rat

    Omim: 604378 Human

    SwissProt: Q14457 Human

    SwissProt: O88597 Mouse

    SwissProt: Q91XJ1 Rat

    Unigene: 716464 Human

    Unigene: 178947 Mouse

    Unigene: 2776 Rat



    BECN1是一種分子質量為60KDa的蛋白質, 它能與細胞凋亡抑制劑Bcl-2相互作用, 減少中樞神經系統的凋亡,參與PI3K復合物的組成和自噬體的形成。主要表達在胞質。抑癌基因beclin 1通過自噬作用來調節細胞生長。
    產品圖片
    Sample:
    Lane 1: 293T (Human) Cell Lysate at 25 ug
    Lane 2: Beclin 1 knockout (KO) 293T (Human) Cell Lysate at 25 ug
    Primary: Anti-Beclin 1 (bs-55024R) at 1/1000 dilution
    Secondary: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution
    Predicted band size: 55-60 kD
    Observed band size: 60 kD
    Sample:
    Lane 1: Cerebrum (Mouse) Lysate at 40 ug
    Lane 2: Small intestine (Mouse) Lysate at 40 ug
    Lane 3: Lung (Mouse) Lysate at 40 ug
    Lane 4: Heart (Mouse) Lysate at 40 ug
    Lane 5: Cerebrum (Rat) Lysate at 40 ug
    Lane 6: Jurkat (Human) Cell Lysate at 30 ug
    Lane 7: Raji (Human) Cell Lysate at 30 ug
    Lane 8: Hela (Human) Cell Lysate at 30 ug
    Lane 9: A431 (Human) Cell Lysate at 30 ug
    Lane 10: HepG2 (Human) Cell Lysate at 30 ug
    Primary: Anti-Beclin 1 (bs-55024R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 55-60 kD
    Observed band size: 60 kD
    Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Beclin 1) Polyclonal Antibody, Unconjugated (bs-55024R) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    Paraformaldehyde-fixed, paraffin embedded (human stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Beclin 1) Polyclonal Antibody, Unconjugated (bs-55024R) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Beclin 1) Polyclonal Antibody, Unconjugated (bs-55024R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    Paraformaldehyde-fixed, paraffin embedded (rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Beclin 1) Polyclonal Antibody, Unconjugated (bs-55024R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    Paraformaldehyde-fixed, paraffin embedded (Mouse small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Beclin 1) Polyclonal Antibody, Unconjugated (bs-55024R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Beclin 1) Polyclonal Antibody, Unconjugated (bs-55024R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (KO Validated)Beclin 1 polyclonal Antibody, Unconjugated (bs-55024R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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